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Community-acquired methicillin-resistant Staphylococcus aureus carrying SCCmec type IV and V isolated from healthy children attending public daycares in northeastern Brazil BJID
Carvalho,Suzi P. de; Almeida,Jéssica B. de; Andrade,Yasmin M.F.S.; Silva,Lucas S.C. da; Oliveira,Arianne C. de; Nascimento,Flávia S.; Campos,Guilherme B.; Oliveira,Márcio V.; Timenetsky,Jorge; Marques,Lucas M..
Abstract Nasal colonization with methicillin-resistant Staphylococcus aureus (MRSA) have increasingly been reported in healthy communities. This study aimed to assess the rate of S. aureus in general and MRSA in particular from nasal secretion of children in daycare centers in Vitória da Conquista, Brazil. The isolates were identified based on morphology, biochemical tests and by PCR. Detection of virulence genes, biofilm production, and susceptibility test by disk diffusion agar were performed. MRSA isolates were characterized by spa, SCCmec, and multilocus sequence typing (MLST). S. aureus were recovered from 70 (47.3%) of 148 children. Among the 11 MRSA strains (15.7%), two SCCmec types (IV and V) were detected. MLST identified four STs related to three...
Tipo: Info:eu-repo/semantics/other Palavras-chave: CA-MRSA; Antibiotic resistance; SCCmec; MLST.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000400464
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Intra-uterine experimental infection by Ureaplasma diversum induces TNF-α mediated womb inflammation in mice Anais da ABC (AABC)
Silva,Jamile R.; Ferreira,Lício F.A.A.; Oliveira,Percíllia V.S.; Nunes,Ivanéia V.; Pereira,Ítalo S.; Timenetsky,Jorge; Marques,Lucas M.; Figueiredo,Tiana B.; Silva,Robson A.A..
Ureaplasma diversum is an opportunistic pathogen associated with uterine inflammation, impaired embryo implantation, infertility, abortions, premature birth of calves and neonatal pneumonia in cattle. It has been suggested that the intra-uterine infection by Ureaplasma diversum can cause vascular changes that hinder the success of pregnancy. Thus, the aim of this study was to evaluate the changes of intrauterine site of A/J mice in estrus or proestrus phase inoculated with Ureaplasma diversum. The infection was monitored at 24, 48 and 72 hours by the PCR methodology to detect the Ureaplasma in the inoculation site and the profile of circulating blood cells. Morphological changes, intensity of inflammation and the production of cytokines were compared. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycoplasma; Uterus; Ureaplasma; Mice.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000200643
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Isolation, pathogenicity and disinfection of Staphylococcus aureus carried by insects in two public hospitals of Vitória da Conquista, Bahia, Brazil BJID
Oliveira,Pollianna S.; Souza,Simone G.; Campos,Guilherme B.; Silva,Danilo C.C. da; Sousa,Daniel S.; Araújo,Suerda P.F.; Ferreira,Laiziane P.; Santos,Verena M.; Amorim,Aline T.; Santos,Angelita M.O.G.; Timenetsky,Jorge; Cruz,Mariluze P.; Yatsuda,Regiane; Marques,Lucas M..
Currently, hospital infection is a serious public health problem, and several factors may influence the occurrence of these infections, including the presence of insects, which are carriers of multidrug-resistant bacterial species. The aim of this study was to isolate staphylococci carried by insects in two public hospitals of Vitoria da Conquista, Bahia and to identify the resistance profile, pathogenicity and efficacy of disinfection of the premises. A total of 91 insects were collected in 21 strategic points of these hospitals, and 32 isolated strains ofStaphylococcus aureus were isolated. Based on antibiogram and Minimum Inhibitory Concentration results, 95% of these strains were susceptible to oxacillin. These strains were also evaluated for the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Insects; Staphylococcus aureus; Resistance; Virulence factors; Disinfection.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000200129
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Molecular characteristics of methicillin-resistant Staphylococcus aureus isolates from hospital and community environments in northeastern Brazil BJID
Carvalho,Suzi P. de; Almeida,Jéssica B. de; Andrade,Yasmin M.F.S.; Silva,Lucas S.C. da; Chamon,Raiane C.; Santos,Kátia R.N. dos; Marques,Lucas M..
ABSTRACT This study characterized 30 MRSA isolates from intensive care unit (ICU) environment and equipment surfaces and healthy children. The SCCmec types I, IVa and V were detected in HA-MRSA isolates while CA-MRSA showed the SCCmec type IVa and V. Most isolates were classified as agr group II. All isolates presented the sei gene, and only HA-MRSA were positive for etb e tst genes. Three genotypes were related to Pediatric (ST5/SCCmecIV) and Berlin (ST45/SCCmecIV) clones. The present study showed molecular similarity between CA- and HA-MRSA isolates in hospital and community settings in a Brazilian region.
Tipo: Info:eu-repo/semantics/other Palavras-chave: Methicillin-resistant Staphylococcus aureus; CA-MRSA; HA-MRSA; Molecular typing.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000200134
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Molecular characterization of ureaplasmas isolated from reproductive tract of goats and sheep from Brazil BJM
Oliveira,Rosângela C.; Yamaguti,Maurício; Marques,Lucas M.; Buzinhani,Melissa; Rizzo,Huber; Meira-Junior,Enoch Brandão de Souza; Guimarães,Ana Márcia S.; Gregory,Lilian; Timenetsky,Jorge.
Abstract Ovine/caprine ureaplasmas have not yet been assigned a species designation, but they have been classified into nine serotypes. Herein ureaplasmas were searched for in 120 samples of vulvo vaginal mucous from sheep and 98 samples from goats at 17 farms. In addition, semen samples were collected from 11 sheep and 23 goats. The recovered ureaplasma were from sheep and goats from animals without any reproductive disorder symptoms, but not all animals presented positive cultures. In sheep, 17 (68%) cultures of vulvovaginal mucous were positive for ureaplasma and 11 (27%) samples of semen presented positive cultures in animals with clinical signs of orchitis, balanoposthitis or low sperm motility. In goats four ureaplasma isolates were obtained from...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ureaplasma; Ovine; Caprine; Molecular characterization.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300560
Registros recuperados: 5
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